Small RNA sequencing and assembly: reconstructing viral genomes from defence molecules

author: Jan Kreuze, International Potato Center (CIP)
published: March 7, 2013,   recorded: February 2013,   views: 3908
Categories

Slides

Related content

Report a problem or upload files

If you have found a problem with this lecture or would like to send us extra material, articles, exercises, etc., please use our ticket system to describe your request and upload the data.
Enter your e-mail into the 'Cc' field, and we will keep you updated with your request's status.
Lecture popularity: You need to login to cast your vote.
  Delicious Bibliography

Description

RNA silencing constitutes a fundamental antiviral defence mechanism in plants in which host enzymes cut viral RNA into pieces of 20-24 nucleotides. When isolated, sequenced en mass and properly assembled or aligned these virus-derived small RNA (sRNA) sequences can reconstitute genomic sequence information of the viruses being targeted in the plant. This approach is independent of the ability to culture or purify the virus and does not require any specific amplification or enrichment of viral nucleic acids as it automatically enriches for small RNAs of viral origin by tapping into a natural antiviral defence mechanism. Results from the application of this technique to identify novel plant viruses as well as map variability and distribution of viruses will be presented.

See Also:

Download slides icon Download slides: nib_kreuze_sequencing_01.pdf (5.0 MB)


Help icon Streaming Video Help

Link this page

Would you like to put a link to this lecture on your homepage?
Go ahead! Copy the HTML snippet !

Write your own review or comment:

make sure you have javascript enabled or clear this field: